By David J. Kyle, Colin Ratledge
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The HPLC purification of ARA is made considerably simpler by the initial urea adduction step because this removes linoleate, which nearly coelutes with ARA under our chromatographic conditions. Greater than 99% purity of ARA is generally required when it is used for the synthesis of prostaglandins, since any contaminating polyunsaturated fatty acid impurities can inhibit the reaction steps involved or make purification of the final product very difficult. In the present work, experiments describing the cultivation of M.
Industrial applications of single cell oils by David J. Kyle, Colin Ratledge