Steven M. Powell's Colorectal Cancer: Methods and Protocols PDF

By Steven M. Powell

ISBN-10: 0896037673

ISBN-13: 9780896037670

ISBN-10: 1592590845

ISBN-13: 9781592590841

Instructs investigators in key genetic, mobile, and molecular organic tools of examining colorectal tumors. concepts and assays are defined intimately to permit researchers to test on colon tumors. such a lot chapters are written through scientists who pioneered the equipment offered. insurance comprises gross microdissection of colon tumors, institution of telephone traces and xenografted tumors, chromosomal analyses resembling FISH assays, mutational analyses of colon tumors and blood samples, gene expression analyses, and microsatellite instability trying out. features a few colour pictures. For researchers at a number of degrees, from graduate scholars to execs. Powell is affiliated with the college of Virginia future health method.

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3. Sequencing apparatus and power supply (Stratagene, CA). 8. Fluorography 1. Large blocking paper (GBM002, Schleicher and Schuell). 2. –80°C freezer. 3. Kodak X-Omat film and a film processor. 3. 1. Tissue Microdissection and Processing 1. For each case to be analyzed use 2–5 H&E stained slides. Examine each slide under a standard microscope to determine the size, the relative position, and the histology of the tumor. Identify regions with the highest tumor components. Use a black Sharpie pen to demarcate the tumor if needed.

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42, 87–98. 4. Tkachuk, D. , and Gray, J. W. (1991) Clinical applications of fluorescence in situ hybridization. Genet. Anal. Techn. Appl. 8, 67–74. 5. , Raap, A. , and Lipp, M. (1992) Specific metaphase and interphase detection of the breakpoint region in 8q24 of Burkitt lymphoma cells by triple-color fluorescence in situ hybridization. Genes Chrom. Cancer 4, 69–74. 6. , Green, E. , and Cremer, T. (1992) Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification. Genomics 13, 826–828.

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Colorectal Cancer: Methods and Protocols by Steven M. Powell


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