By Harold E. Himwich
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In the other procedure (Kakimoto and Armstrong, 1962), amines are adsorbed on to the sulfonic acid cation exchange resin, Dowex 50Wx2, in the hydrogen form, and the resin is washed with 0-1 N sodium acetate and water, and the adsorbed bases are then eluted with 1 N ammonium hydroxide in 6 5 % ethanol. The alcoholic ammonia eluate is taken to dryness on a rotary evaporator, and after desalting by ethanol extraction is subjected to chromatography. In each of these procedures, both the free urinary amines are studied, as well as those conjugated amines not originally adsorbed on to the resin, which are freed by subsequent acid hydrolysis and then adsorbed on to fresh resin.
After elution of the appropriate areas with chloroform, the acids were esterified by short treatment with diazomethane in ether. Studies with known compounds revealed that n o methylation of phenolic groups occurred under these conditions. G a s chromatography, using a column of neopental glycol succinate adsorbed on gas chrom P, afforded a n excellent resolution of 3,4-dimethoxy as well as the isomeric mono-O-methyl dihydroxyphenylacetic acids. By addition of 3,4-dimethoxyphenylacetic acid t o urine, it was possible to detect less than 1 fig per gram creatinine output.
P . , GALE, P . H . , 2 0 3 , 154. K U E H L , F . A . , V A N D E N H E U V E L , W . J. A . , a n d O R M O N D , R . E . (1966) t o be published. M I C H A U X , R . a n d V E R L Y , W . G . (1963) Life Sciences, p . 175. O R M O N D , R. E . , M A N D E L , L. R . a n d K U E H L , F . A . , Jr. (1965) u n p u b l i s h e d observations. P E R R Y , T . L . , H A N S E N , S . a n d M A C I N T Y R E , L. (1964) Nature, PORTER, C . C . (1964) unpublished results. S E N , N . P . and M C G E E R , P .
Amines and Schizophrenia by Harold E. Himwich